Lucas Pelkmans, UTH Zurich: Population context predicts activity in human cells
Ulrike Eggert, Harvard Medical School: A combined chemical genetic/RNAi approach: new tools to study cell division
Charlie Boone, Univ. of Toronto: Integrating high-throughput yeast genetics and high content screening
Jonathan Weissman, UCSF: Biology without basis: functional insights from high-resolution genetic interaction maps
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2 comments:
can you tell me the paper on the ribosomal rnase protection assay
Yael
Hey yael, I don't know which paper introduced this assay but I think it's an old one... Basically you just digest all the mRNA in the cell with RNAse and the ribosomes will protect the actively translated sequences. Weissman was applying RNA-SEQ to all these actively transcribed sequences in the prion-treated cells vs. controls, thus generating a list of actively transcribed mRNAs.
I don't think the work is published yet, but you could e-mail him for the reference :)
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